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Course Contents

Theory of electronic absorption spectra, Beer's law, Absorption cross-section, Solvatochromism (4)

Radiative and non-radiative transitions, Vibrational relaxation, Internal conversion, Inter system crossing (5)

Fluorescence, Phosphorescence, Various photophysical processes, Solvent effect on emission, Lippert equation, Dynamic stokes shift, Dynamic and static quenching, Stern-Volmer equation, Forster resonance energy transfer and theory, Fluorescence anisotropy and Perrin equation, Excited state proton/electron transfer, Excimer and exciplex  (20)

Laser fundamentals, Stimulated emission, Population inversion, Light amplification, Pulsed laser: cavity dumping, Q-swithching, mode-locking (4)

Spectroscopic techniques, Spectrophotometer, Spectrofluorimeter, Time correlated single photon counting, Transient absorption, Streak camera, Fluorescence up-conversion (6)

Single molecule spectroscopy, Fluorescence correlation spectroscopy, Protein fluorescence (3)