Ishita Sengupta

PhD (The Ohio State University)

Assistant Professor, Department of Chemistry

Specialization

 

Education

  • PhD (The Ohio State University)

Website(s)

CV

We are broadly interested in studying protein structure and dynamics using biomolecular NMR spectroscopy. In parallel, we will also investigate the physical and conformational changes accompanying complex biomolecular reactions (e.g. protein aggregation) using a variety of biophysical tools. The laboratory will work at the intersection of physical chemistry and structural biology of proteins, using a combination of NMR and other spectroscopic tools, with the help of modern molecular biology and protein biochemistry techniques.

In particular, we will be investigating how long-range interactions (tertiary structure) can tune the local secondary structure of proteins, with the ultimate goal of designing proteins which can reversibly adopt more than one unique fold. This idea has parallels with protein aggregation reactions, which are also often accompanied by drastic conformational changes. More specifically, transient conformational fluctuations in proteins have been hypothesized to initiate protein aggregation and misfolding reactions. We aim to detect these high energy states, modulate their stabilities and lifetimes by protein engineering and binding, and ultimately solve their structures in both the native and the aggregated states.

Motivated individuals with a background in biomolecular NMR spectroscopy and/or protein biophysics are encouraged to apply. Please contact me at This email address is being protected from spambots. You need JavaScript enabled to view it. with a CV and a short description of your research interests.

  • Protein fold determined by paramagnetic magic-angle spinning solid-state NMR spectroscopy, Ishita Sengupta, Philippe S. Nadaud, Jonathan J. Helmus, Charles D. Schwieters and Christopher P. Jaroniec, Nature Chemistry, 4, 410–417 (2012)
  • Protein Structure Determination with Paramagnetic Solid-State NMR Spectroscopy, Ishita Sengupta, Philippe S. Nadaud, and Christopher P. Jaroniec, Accounts of Chemical Research, 46 (9), 2117–2126 (2013)
  • Salt-mediated oligomerization of the mouse prion protein monitored by real-time NMR, Ishita Sengupta, Suhas H. Bhate, Ranabir Das, and Jayant B. Udgaonkar, Journal of Molecular Biology, 429 (12), 1852-1872 (2017)
  • Monitoring site-specific conformational changes in real-time reveals a misfolding mechanism of the prion protein, Ishita Sengupta and Jayant B. Udgaonkar, eLife, 8, e44698 (2019)

  • Assistant Professor, IIT Kanpur, 2018

  • Postdoctoral Fellow, National Centre for Biological Sciences, 2013-2018

Office

FB 437,
Department of Chemistry
IIT Kanpur,
Kanpur 208016

Office Phone: 0512-259-2061

Email: ishita[AT]iitk.ac.in

   
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