Facility for Transgenesis of Multiple Model Organisms

 

Transgenic techniques have revolutionized biological science research. This technique allows scientists to introduce or eliminate a gene of choice from an organism to interrogate the role of the gene in the physiology of the animal. BSBE department had infrastructure to conduct such experiments in worms, flies and birds but not in fish or mice. The equipments obtained through the CARE proposal (2012-13) lays the foundation to eventually achieve these.

The acquired facility integrates two platforms which together makes transgenesis (genetic manipulation) in fish and mice feasible: namely,

  • A setup for in vivo manipulation (a set of high-end stereo binocular Microscopes with fluorescence and imaging accessories) and

  • Post-transgenesis care and rearing of genetically modified organisms.

The items under component (1) is a set of three stereo-binocular microscope while those under component (2) includes set up for (a) aquaculture with sanitized, temperature- and oxygen-regulated circulated water system and culture tanks for Zebrafish and (b) individually-ventilated caging system for post-operative sterile housing of the transgenic animals.

Location

Mouse Facility
First Floor,
BSBE (Biological Sciences &
Bioengineering) Building

Zebra Fish Facility
Basement,
BSBE (Biological Sciences &
Bioengineering) Building

Contact

Mouse Facility
Prof. Amitabha Bandyopadhyay
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Zebra Fish Facility
Prof. Pradip Sinha
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Photograph showing the individually ventilated cage system and the ventilator installed in the BSBE laboratory to house transgenic mice