About Samples
Because SEM utilizes vacuum conditions and uses electron beam to form an image, special sample preparations is needed as listed below:
- The sample is mounted on carbon tape that is put on the surface of the stub.
- Approximate size of the sample 1 x 1 cm.
- All water must be removed from the samples because the water would vaporize in the vacuum.
- Electrically conductive samples do not require any preparation before being used, however the samples must be completely dry and free from volatile substances.
- Non-conductive samples need to be made conductive by coating the sample (using sputter coater) with a thin layer of conductive material like Au/Ag/C.