Stem Cell Research Facility
Deep Freezer:
Deep freezers are used to store reagents, antibodies and biological samples like blood and serum etc. for a long period of time. Deep freezers can store samples below -40℃ (-40℉).
Refrigerator:
Biological reagents like media, growth factors and antibodies should be keep at or below 4°C.
Bio cabinet (Class II):
The Class II Biosafety Cabinet is mainly used for cell culture that requires a sterile work zone. It has HEPA filter that helps in preventing contamination.
CO2 Incubator:
A CO2 incubator is used to maintain an optimal environment for cell growth. It provides the optimum temperature; moisture (sterile environment) and helps in maintain the optimum pH of media.
Table centrifuge/ refrigerated centrifuge:
Tabletop centrifuges are used for making cell pallet or separating particulates in suspension. Refrigerated centrifuge are used for isolation of RNA from cell and tissues at 14000 rpm.
Shaker:
A shaker is a piece of laboratory equipment used to mix, blend, or agitate substances in a tube or flask by shaking them.
Microplate reader:
Microplate reader is the next generation 8-channel absorbance microplate reader by Bio-Rad. Its applications range from basic ELISA to high throughput detection.
Fume Hood:
A fume hood is used typically to prevent the release of hazardous substances into the general laboratory space by controlling and then exhausting hazardous and/or odorous chemicals.
Fluorescent Microscope:
A fluorescence microscope is an optical microscope that uses fluorescence to generate an image. Fluorescence is a form of luminescence that through microscopy allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Co-localization and interaction studies can be performed, and intercellular processes like endocytosis and exocytosis can be observed.
qPCR
Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified organisms in food, and cancer phenotyping.
Mini submarine Gel system:
Agarose Gel electrophoresis is a technique which is used for the analysis of DNA and RNA. In this technique, DNA or RNA is loaded into a semi-solid gel phase made of agarose. When an electric field is applied across the gel, the biomolecules move to the oppositely charged electrode. In this process, the fragments of the DNA or RNA separate according to their sizes and molecular weight to give distinct bands on the gel. The gel is placed in a tank containing an electrophoresis buffer.
Gel Doc Ez Imager:
The Gel Doc EZ is a compact and automated imaging system for obtaining publication-quality images and analyzed data. The system provides unparalleled flexibility with the use of four application-specific trays, which include a UV tray (for ethidium bromide staining of DNA gels and fluorescence imaging), a white tray (for Coomassie, copper, silver, and zinc stains), a blue tray (for nucleic acid applications that use SYBR® stains), and a stain-free tray for stain-free imaging.