Live Cell Imaging Facility with Advanced High Sensitive Spectral Confocal and Multiphoton Microscope System

Carl Zeiss LSM780 NLO microscopy system with Tunable laser from Coherent. System supplied by Carl Zeiss India Pvt Ltd.

 

 
 

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This facility includes following components: A high-end inverted fluorescence microscope with onstage incubator for cell culture and programmable/motorized control features (X, Y and Z scans) for capturing bright field and fluorescence imaging (for DAPI, rhodamine, TRITC, FITC, GFP, YFP, and CFP) using a high resolution peltier cooled monochrome camera. The system is supported with tunable femtosecond laser source (tuning range: 690-1040 nm) with PMT and GaAsP modules for signal detection. The system is capable of simultaneous acquisition and separation of 10 fluorophores and includes control software modules for controlling all motorized components of microscope, laser scan head, laser module, stage incubation system and the digital camera. The facility in addition has incubator and biosafety cabinet for handling of mammalian cell and tissue cultures.

Contact person

Jonaki Sen

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Research
Confocal, microscope, live-cell imaging, multiphoton.
Links for videos
Room 209, ACMS, IIT Kanpur
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