Prerequisites:
CHM 481
Course Contents:
Enzyme kinetics of single and multiple substrate systems including Enzyme assays and inhibition
(15 lectures)
Cooperativity and multienzyme systems (4 lectures)
Enzyme structure and identification of active site residues labelin, chemical modification and mutagenesis
(6 lectures)
Enzyme Mechanisms – Methods of study and mechanisms of some enzymes like
Serine proteases, polymerases, ribonucleases, lysozyme and ribonucleotide reductases (radical enzyme)
(15 lectures)
Mechanism based enzyme inhibition and drugs –5-fluorouracil for thymidylate synthase
(2 lectures)
